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Inviting applications for a training program on'CRISPR/Cas9-based Gene Editing Technologies in Plant

Title: 'CRISPR/Cas9-based Gene Editing Technologies in Plants' to be held from 12 - 16th Jun 2023

BioNcube, a BIRAC-Bio incubator of the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) calls for applications for a hands-on training program on “CRISPR/Cas9 Based Gene Editing Technologies in Plant”. ICRISAT is an International non-profit agricultural research institute with state-of-the-art facilities for Agri-biotechnology research and product translation.



Course description

The cutting-edge tools and technologies currently available to breeders are collectively known as Novel Breeding Technologies (NBT) of which Gene Editing (GE) is the foundation. GE permits rapid and precise trait creation and selection without negative consequences on the suite of native phenotypes. These methods could accelerate genetic discovery, development and product delivery, enabling ” reductions” in farmers’ input costs and “exponential improvements” in the agronomic and nutritional value of food and output. For researchers wishing for a well-rounded theoretical and practical introduction to gene editing, this hands-on training program on ” CRISPR/Cas9-based Gene Editing Technologies in Plants” is ideal. The training program comprises is aimed at providing a better understanding of various elements and stages of gene editing.


This course will familiarize the participants with gene BioNcube @ ICRISAT editing workflows, from the design and synthesis of target-specific guide RNAs (gRNAs), demonstration of delivery of gRNAs in plant cells, and detection and analysis of mutations.


This is an excellent opportunity to interact and benefit from the expertise of our scientists and collaborators as they will hand-hold the trainees to design their first gene editing experiment and gene editing workflows.

We are confident that the training program will enable the participants to develop their experimental workflows.



Objective

  • the concepts of gene editing

  • designing of guide-RNAs and CRISPR/Cas9 constructs

  • usage of high-throughput techniques for gene editing applications

  • methodology for molecular and functional validation of edited lines

  • ethics and biosafety of gene editing technologies


The training course is being organized from June 12th – 16th at ICRISAT, Patancheru, Hyderabad, India-502 324


The final date for application is 30 May 2023










Lectures

  • Genome Editing technologies - applications and future prospectives

  • Editing of crop genomes for trait development: new directions and challenges

  • Development of biotic and abiotic stress tolerance through CRISPR/Cas9-based genome editing system and functional genomics approach

  • Crop improvement using a genome-editing approach.

  • New breeding tools for accelerated breeding in dryland cereals




Practical sessions

  • Bioinformatics tools for gRNA(s) design

  • Strategy and cloning of the gRNA(s) and Cas9 in the plant expression vectors

  • Agrobacterium transformation for the CRISPR/ Cas9 recombinant plasmids by electroporation

  • Demonstration of delivery of CRISPR cassettes into plant cells

  • Identification of mutations

  • Molecular analysis of the gene-edited plants



Application Form: Click here


https://www.icrisat.org/event/hands-on-training-program-on-crispr-cas9-based-gene-editing-technologies-in-plants/






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